Abstract : The objective of the study was to assess the conservative effects of L-Ergothioneine (EGT), antioxidant, on the post thaw chromatin integrity of human spermatozoa. 400 normozoospermic semen samples were cryopreserved with (Test) and without (Control) supplementation of EGT. Clinically relevant tests such as Semen analysis (WHO 2010), Sperm chromatin integrity (Sperm chromatin dispersion test) and Lipid peroxidation status (Malondialdehyde concentration assessment) were performed in both fresh and frozen thawed samples. It was seen that with the increase in ROS production due to cryopreservation, there is a significant decrease in the post thaw sperm parameters and sperm chromatin integrity (Fresh vs Control: p<0.001), the extent of which was reduced on addition of EGT (Test vs Control: p<0.001). Also interestingly, the increasing ROS levels (assessed in MDA test) correlated directly with DNA damage. Hence the study provides a basal proof that EGT acts against the ROS and conserves the spermatozoa's functionality.
Abstract : Diabetes mellitus (DM) is a disorder recognized with increase of blood sugar level. Chronic hyperglycemia leads to the dysfunction of several organs especially eye, kidney, heart and blood vessels. DM eventually leads to the peripheral nervous system complications like diabetic neuropathy, in that especially neuropathic pain is the major presentation. The aim of this study was to investigate the effect of Asystasia gangetica and Morus indica in the treatment of diabetic neuropathy in alloxan induced diabetic mice. Diabetes was induced in the male albino mice weighing 20- 30 gm by intraperitoneal injection of alloxan. Four weeks after alloxan injection animals were divided into six groups as control (I), standard (II) and 4 experimental groups (III, IV, V, VI). Group I received normal saline and group II received intraperitoneal injection of standard diclofenac sodium (10mg/kg). The experimental groups III, IV and V, VI received oral different doses of ethanolic extract of A.gangetica (50,100 mg/kg) and Morus indica (100,200 mg/kg) respectively. Blood glucose and phytochemical analysis were performed by using standard kits. Phytochemical analysis reveals the presence of flavanoids and antioxidants. Neuropathic pain assessed by tail immersion, hot plate and electrical stimulation of tail methods. All the experimental groups shows significant increase in response time when compared to control groups. Overall, Asystasia gangetica and Morus indica exerted analgesic effect in neuropathic mice.