- N B Sridharamurthy* ,
- Ankita Chatterjee ,
- Channaveeraswamy THM ,
- Vishnupriya K
Abstract : The present investigation was under taken to study the evaluation of proconvulsivepotentials of levofloxacin a fluoroquinolonein epilepsy induced models like PTZ, Theophylline at sub convulsive dose and by electroconvulsometer; MES.Convulsions can be induced by electric shock of 150mA for 0.2 sec using ear clip electrodes. The duration in seconds of tonic flexion, tonic extension, clonic convulsion and post titanic depression can be noted. The increase in the duration of tonic extensor phase was considered as epileptic index. The convulsion can also be induced by giving sub convulsive dose i.e. 40mg/kg of PTZ intra-peritonealy. The animals were kept under observation for 15mins for the development of jerky movements and clonic seizures. Each mouse under the test received the test drug intraperitoneally 30 mins before i.p administration of subconvulsive dose of theophylline (125mg/kg). The animals were kept under observation for the onset of maximal seizures which was evidenced by tonic flexion of fore limbs, tonic extensor of hind limbs and clonic convulsions for 2 hours. The pro-convulsive potentials of Levofloxacin at two different doses were studied using MES, PTZ methods and co administered with theophylline and the results were compared with the control group and standard group. The results of the present study showed that levofloxacin demonstrated a significant proconvulsive profile in both MES and PTZ induced seizures and thus should be prescribed with caution. The % decrease in the level of GABA treated with levofloxacin at 25 mg/kg bodyweight in mice brain is 10.92 as compared to control was 16.14 in MES induced seizures.The %decrease in the level of GABA treated with levofloxacin at 25 mg/kg bodyweight in mice brain was 7.85 as compared to control was 16.14 on PTZ induced seizures. Similarly the GABA level was also decreased to 8.88% on treatment with levofloxacin at 25mg/kg bodyweight as compared to control was 16.14% on co administration withtheophylline.
Keyword : Levofloxacin, PTZ, MES, Theophylline, proconvulsive activity, GABA.