Abstract : Keratins are the most abundant proteins in epithelial cells of vertebrates. The keratinise wastes are produced in large quantities all over the world from animals and birds including human beings. Worldwide poultry processing plants are producing millions of tons of feathers as waste product annually, which consists of approximately 90% keratin. The present study investigated keratinises from the decaying poultry feather and human hair samples. Two types of indigenous fungi were isolated and their enzyme activity was measured. The fungi were named as Aspergillus sp and pencillium sp. These fungi were then tested for the production of amylase, protease, lipase and keratinase. Both the fungi were to produce amylase, protease and keratinase. The extracellular lipase was more found to be Aspergillus sp when compared to pencillium sp. Substrate decomposition was found to be better in Aspergillus sp than in pencillium sp. Aspergillus sp showed 38% loss of hen feather as compared to 25% loss of human hair whereas pencillium sp showed 21% loss of feather and 20% of human hair. Two fungi were tested for the production of amino acid by thin layer chromatography. Comparatively, Aspergillus sp was found to be producing more amounts of enzymes.